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应用活体流式细胞仪研究肝癌肿瘤细胞转移
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Circulation times of hepatocellular carcinoma cells by in vivo flow cytometry

摘    要
肝癌具有转移性,会侵袭其它器官,而肝癌转移患者的治疗后生存率非常低。因此肝癌转移过程需要更详细和深入的研究,从而找到更好的治疗方法。我们结合“在体共聚焦荧光显微镜”和流式细胞仪的概念发展了“活体流式细胞仪”技术,来研究微环境内肿瘤细胞的播散过程;具体在本文中,研究肝癌转移细胞在循环系统内的播散与动力学特性。我们测量了两种来自同一肝癌病人但经过筛选后具有不同转移潜能的肝癌细胞株的血行播散动力学,分别为具有高转移潜能的HCCLM3 细胞和具有低转移潜能的HepG2 细胞。经静脉注射1小时后, 大于60%的HCCLM3 肝癌高转移细胞从血循环中消失。与此对比,HepG2肝癌低转移细胞在血循环中消失速度明显较慢;经静脉注射1小时后,不到40%的HepG2肝癌低转细胞从血循环中消失。血行播散的无创连续监测有助于我们研究肿瘤转移的早期过程。
标    签 活体流式细胞仪,肝癌转移,循环肿瘤细胞,近红外,共聚焦成像   170.0170   170.3880   170.4580  
 
Abstract
Hepatocellular carcinoma (HCC) may metastasize to many organs. The survival rate is almost zero for metastatic HCC patients. Molecular mechanisms of HCC metastasis need to be understood better and new therapies must be developed. We have developed the "in vivo microscopy" to study the mechanisms that govern liver tumor cells spreading through the microenvironment in vivo. A recently developed "in vivo flow cytometer" combined with real-time confocal fluorescence imaging is used to assess spreading and the circulation kinetics of liver tumor cells. We measure the depletion kinetics of two related human HCC cell lines, high-metastatic HCCLM3 cells and low-metastatic HepG2 cells, which are from the same origin and obtained by repetitive screenings in mice. More than 60% of the HCCLM3 cells are depleted within the first hour. Interestingly, the low-metastatic HepG2 cells possess noticeably slower depletion kinetics. In comparison, less than 40% of the HepG2 cells are depleted within the first hour. The differences in depletion kinetics might provide insights into early metastasis processes.

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收稿日期 2010/7/7

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备注This work was partially supported by the National "973" Program of China (No. 2011CB910400), the China National Key Projects for Infectious Disease (No. 2008ZX10002-021), the National Science Foundation of China (Nos. 30770524 and 20975027), the Ministry of Education of China (No.109056), "Shuguang Scholarship" from the Education Commission of Shanghai Municipality, and the Program for New Century Excellent Talents in University Award (NCET-08-0131).

引用该论文: Yan Li,Zhichao Fan,Jin Guo,Guangda Liu,Xiaoying Tan,Cheng Wang,Zhengqin Gu,Xunbin Wei. Circulation times of hepatocellular carcinoma cells by in vivo flow cytometry[J]. 中国光学快报, 2010, 8(10): 953~956


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