光谱学与光谱分析, 2018, 38(9): 2843, 网络出版: 2018-09-01
Method for Detecting Pesticide Content of Carbofuran by Fluorescence Spectrometry
燕山大学测试计量技术及仪器河北省重点实验室, 河北 秦皇岛 066004
农药的广泛使用是防治病虫害的一种必要手段, 在保证农业增产增收的同时, 也给人们健康带来了威胁, 寻求更加灵敏的农药残留检测方法至关重要。 三维荧光光谱分析法选择性好、 灵敏度高、 快捷、 取样少, 可用于农药残留的分析与检测中。 以自制标准浓度的克百威农药溶液作为测试样本, 乙腈为溶剂, 利用荧光光谱仪对样本进行测量, 将实验获得的三维数据用MATLAB进行处理获得其三维荧光光谱图。 实验发现乙腈作为溶剂对实验影响不大且荧光光谱的强度与克百威溶液浓度呈现良好的线性关系, 相关系数r=0.997 4, 接近于1。 因此三维荧光光谱分析法可以检测出克百威溶液的浓度以及农药的含量。
As a necessary means of preventing and controlling diseases and insect pests, the widespread use of pesticides has brought great threat to the people’s health, so finding some more sensitive methods of pesticide residues is of great significance. Fluorescence spectrometry has the advantages of good selectivity, high sensitivity, quick sampling and less sampling. It can be used in the analysis and detection of pesticide residues. In this paper, Carbofuran of self-made standard pesticide solution concentration as test samples, acetonitrile as solvent were measured on samples by fluorescence spectrometer, 3D datas will be obtained by MATLAB to get the 3D fluorescence spectra. The experimental results show that using acetonitrile as solvent has little effect on the experiment and the intensity of the fluorescence spectrum has a good linear relationship with the concentration of carbofuran solution. The coefficient of correlation R2=0.997 4 and it is close to 1. So it is a feasible and reliable method to detect the carbofuran pesticide with the three-dimensional fluorescence spectrometry.