光学学报, 2003, 23 (4): 451, 网络出版: 2006-06-27
光纤倏逝波生物传感器及其应用
Fiber-Optic Evanescent Wave Biosensor and Its Application
光学计量 传感器技术 生物传感器 倏逝波 光纤 核酸 optical metrology senser technology biosensor evanescent wave optical fiber nucleic acid
摘要
以光纤倏逝波原理,研制成功一台光纤倏逝波生物传感器。该传感器对纯净的Cy5荧光染料溶液的检测灵敏度达0.01 μmol/l;对浓度为0.01 μmol/l的嗜肺军团菌核酸样品的测量信噪比为4.61。所有检测结果与商品化生物芯片扫描仪同时检测得到的结果具有很好的一致性。
Abstract
A fiber-optic evanescent wave biosensor has been developed based on the principle of evanescent wave while light propagates in optical fiber. Sensitivity limit of 0.01 nmol/l is obtained from the detection of Cy5 serial solutions with various concentrations. And an ideal result of signal-to-noise ratio of 4.61 is obtained when the biosensor is used to measure Legionella pneumophila solution of 0.01 μmol/l. All the results are comparable with that obtained by a commercial biochip scanner.
黄惠杰, 翟俊辉, 任冰强, 杨瑞馥, 赵永凯, 程兆谷, 杜龙龙, 路敦武. 光纤倏逝波生物传感器及其应用[J]. 光学学报, 2003, 23(4): 451. 黄惠杰, 翟俊辉, 任冰强, 杨瑞馥, 赵永凯, 程兆谷, 杜龙龙, 路敦武. Fiber-Optic Evanescent Wave Biosensor and Its Application[J]. Acta Optica Sinica, 2003, 23(4): 451.
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