用氯仿、乙醚、乙醇和盐酸等溶剂溶解一组人体胆结石,获取难溶物;用傅里叶变换红外光谱和表面增强拉曼光谱对难溶剩余物进行研究.结果显示,结石难溶物主要由胆红素盐和蛋白质组成,结石中的蛋白质的二级结构以α螺旋和无规卷曲构象为主,其中,α螺旋构象成分较多.讨论了蛋白质在胆结石形成中的作用.

A set of human gallstones were dissolved with chloroform, ethanol, ether and hydrochloric acid, the insoluble remains were analyzed by Fourier transform infrared (FT IR) spectroscopy and surface enhanced Raman (SER) spectroscopy. The results show that the insoluble remains of gallstones contain mainly bilirubinate salts and proteins. The secondary structure of proteins in gallstones is composed mainly of α helix and random coil, in which α helix structure is dominant. The relationship between proteins and gallstone formation is discussed.