采用新型显微荧光分光光度系统，实现激光诱导人肺组织内源性显微荧光光谱与图像的测量，获得不同组织层(即上皮层，粘膜层和软骨层)的荧光分布和荧光特性，为探索癌与正常组织自体荧光光谱特征差异的根源，揭示激光诱导自体荧光法诊断和定位早期肺癌的机理提供重要的实验依据。

The microscopic fluorescence spectra and imaging of excised lung tissue sections were studied under a novel microspectrophotometer system using a He Cd laser light at 442 nm. Differences of the intrinsic autofluorescence distributions in different tissue layers, i.e., epithelium, mucosa and cartilage, were observed in normal and malignant lung tissues. The results show that the study of microscopic fluorescence on various tissue layers can be used to explore the origin of spectral differences between normal and abnormal lung tissues, which is very useful for the better understanding of the mechanism of the early cancer diagnosis using the laser induced autofluorescence technique.