利用光纤倏逝波原理,以输出波长为635 nm的半导体激光器为光源,研制成功一台具有5个探头的光纤生物传感器。该传感器5个光纤探头对纯净Cy5荧光染料溶液的检测灵敏度均达0.01 nmol/L,在同一浓度下信噪比的相对标准偏差小于10%,5个探头的信噪比曲线几乎重合,且与商品化生物芯片扫描仪同时检测得到的结果一致[检测到了抗原抗体特异性反应的动态过程。本传感器具有较高的检测灵敏度、良好的响应一致性和生物特异性,可用于多重生物物质的检测。

A multi-probe fiber-optic evanescent wave biosensor has been developed based on the principle of evanescent wave while light propagates along optical fiber. A laser diode at 635 nm wavelength is used as the light source. The biosensor can interrogate five individual fibers at one time. Thus assays for five separate analytes can be conducted on the same sample. For all the five fibers, sensitivity limit of 0.01 nmol/L is obtained from the detection of Cy5 serial solutions with various concentrations. The response curves of five fibers have good consistency, the relative standard deviation is less than 10%. All the results are comparable with those that are obtained by a commercial biochip scanner. Real-time fluorescence of human IgG binding to goat anti-body IgG antibody immobilized on fiber core has been successfully detected.