基于对处于平衡态少量荧光分子集合的强度涨落进行时间平均的技术,荧光相关谱(fluorescencecorrelationspectroscopy,FCS)技术最近已经应用于细胞环境过程的研究.FCS优秀的灵敏特性为我们实时测量许多参数提供了途径,而且具有快速的时间特性和高空间分辨率.测量的参数包括扩散速率、局部浓度、聚合状态和分子间的相互作用.荧光互相关谱(fluorescencecross-correlationspectroscopy,FCCS)进一步扩展了FCS技术的应用,包括在活细胞中的广泛应用.本文介绍了FCS技术的原理、实验装置及其应用.

Based on time-averaging fluctuation analysis of small fluorescent molecular ensembles in equilibrium, fluorescence correlation spectroscopy (FCS) has recently been applied to investigate processes in the intracellular milieu. The exquisite sensitivity of FCS provides access to a multitude of measurement parameters (rates of diffusion, local concentration, states of aggregation and molecular interactions) in real time with fast temporal and high spatial resolution. The fluorescence cross-correlation spectroscopy (FCCS) has expanded the utility of the technique to encompass a wide range of promising applications in living cells. This paper introduces the theory and application of FCS.