PMF方法由于具有高灵敏度、高通量和容易自动化等优点,在蛋白质组学鉴定中占有重要的地位.然而,许多样品(比如:小分子蛋白,混合物等)仅仅通过PMF方法不能明确鉴定.在这种情况下,在测定PMF的同一个样品上,选择一个酶解片段峰进行PSD测序,并把这些序列信息输入MS-Tag软件进行搜索,结合PMF方法,表观分子量等电点等参数,能够对胶上的点进行明确的鉴定.本文先用PSD方法对胶上的三个标准蛋白进行鉴定,都得到了非常准确的结果,同时鉴定了胶上的几个未知点.

Due to its short analysis time, its high sensitivity and ease of automation, matrix assisted laser desorption/ionization (MALDI)peptide mass fingerprinting has become the important method for identifying proteins of which the sequences are available in databases. However, many protein samples cannot be unambiguously identified by exclusively using their peptide mass fingerprints (e.g., protein mixtures, heavily posttranslationally modified proteins and small proteins). In these cases, additional sequence information is needed and one of the obvious choices when working with MALDImass spectrometry (MS) is to choose for post source decay(PSD) analysis on selected peptides. These sequencing information is imported in MSTag software in order to search in sequencing database .The combination of peptide mass fingerprinting and PSD and analysis described here generally leads to unambiguous protein identification in the amount of material range generally encountered in most proteome studies. We identified three maker protein and three unknown protein from 2D-gel in PSD in the paper.