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碳离子束辐照对质粒DNA的损伤效应

Damage of Plasmid DNA Induced by Carbon Ion Beam

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摘要

为了证明DNA双链断裂(DSB)片段分布与DNA序列有关的假设,采用32keV/μm的12C6+离子和45keV/μm的13C6+离子分别辐照pUC18质粒,结合限制性内切酶处理,进行琼脂糖凝胶电泳,分析DNA断裂和片段分布.结果表明:除了由一个DSB导致的线性DNA带外,还出现了一条新的、小分子量线性DNA带;限制性内切酶处理后,有另一条线性DNA带产生.证明重离子辐照诱导的DSB是非随机分布的,DNA分子上存在对电离辐射相对敏感的位点.

Abstract

To verify our hypothesis of the correlation between DNA sequence and the nonrandom distribution of DNA doublestrand break (DSB), pUC18 was irradiated by 32keV/μm12C6+ ions or 45keV/μm13C6+ ions. Agarose gel electrophoresis and restriction endonuclease were employed to analysis the DNA beaks and their distribution. Dose response and the distribution of DNA fragments were obtained. Besides the linear DNA band induced by one DSB, another linear DNA band was found. After treatment with restriction endonuclease, a new linear DNA band emerged. It is testified that the distribution of DSB fragments is nonrandom. This implies that there may be relatively sensitive sites to ionizing radiation in DNA molecules.

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中图分类号:Q691;Q7

所属栏目:离子束生物工程

基金项目:"西部之光"项目资助(XB980611)

收稿日期:2003-07-05

修改稿日期:--

网络出版日期:2003-10-01

作者单位    点击查看

周光明:中科院近代物理研究所,中国甘肃,兰州,730000
李文建:中科院近代物理研究所,中国甘肃,兰州,730000
冯岩:中科院近代物理研究所,中国甘肃,兰州,730000
李强:中科院近代物理研究所,中国甘肃,兰州,730000
马秋峰:中科院近代物理研究所,中国甘肃,兰州,730000
卫增泉:中科院近代物理研究所,中国甘??兰州,730000
高清祥:兰州大学生物系,中国甘肃,兰州,730000

备注:周光明:男,1970年7月出生于湖北省枝江县.1994年毕业于兰州大学生物系生物化学专业,同年分配到中国科学院近代物理研究所工作,2001年获博士学位,现为助理研究员.目前主要从事重离子束治癌基础研究和重离子辐射生物学效应机理研究工作,已发表学术论文十余篇. 高清祥,通讯联系人

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引用该论文

周光明,李文建,冯岩,李强,马秋峰,卫增泉,高清祥. Damage of Plasmid DNA Induced by Carbon Ion Beam[J]. Acta Laser Biology Sinica, 2003, 12(5): 337-340

周光明,李文建,冯岩,李强,马秋峰,卫增泉,高清祥. 碳离子束辐照对质粒DNA的损伤效应[J]. 激光生物学报, 2003, 12(5): 337-340

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