激光生物学报, 2002, 11 (3): 224, 网络出版: 2006-06-02
HPLC法测定头孢克肟的含量
HPLC Determination of the Content of Celifixne
摘要
采用高效液相色谱法测定头孢克肟的含量,HPLC法C18为填充柱(6.0×150mm),以氢氧化四丁铵 -乙腈(340:160)为流动相;检测波长为254 nm.结果:线性范围为0.144 mg/ml~0.280 mg/ml(r=0.9999),平均回收率为99.80%,RSD=0.20%(n=3) . 本法具有柱效高、经济等优点,优于USP法.
Abstract
Method:To determine the content of celifixine by HPLC, the HPLC system consisted C18 column (6.0×150mm), (340:160) mixture as mobile phase ,with detection at 254 nm.Result: The linear range was 0.144 mg/ml~0.280 mg/ml (r=0.9999),The mean recovery was 99.8%,RSD= 0.20%(n=3). The method is simple,sensitive and accurate.
许玉丽, 王端统, 王腾. HPLC法测定头孢克肟的含量[J]. 激光生物学报, 2002, 11(3): 224. 许玉丽, 王端统, 王腾. HPLC Determination of the Content of Celifixne[J]. Acta Laser Biology Sinica, 2002, 11(3): 224.