Raman spectroscopic studies on the conformational changes of calf thymus DNA induced by Cd2+ ions
We used Raman spectroscopy to study the conformational changes of DNA induced by Cd2+ ions in different Cd2+ concentrations solution. The experimental results show that when the Cd^(2+)/PO^(-)_(2) ratio R increased from 0 to 3.0, the band 835.0 cm^(-1) shifted about 8 cm^(-1), and the overlapping spectra of 1446.0 and 1461.0 cm^(-1) separated and moved to 1441.0 and 1458.0 cm^(-1), respectively. This indicates that the conformation of DNA has changed from a "normal" B-form to a "modified" B'-form. At the same time, changes of other bands demonstrate that parts of base stacking collapse and some hydrogen bonds between AT are disrupted, AT base pairs are damaged more larger than GC base pairs.
Yaoyong Meng：Institute of Laser Life Sciences, South China Normal University, Guangzhou 510631
Songhao Liu：Institute of Laser Life Sciences, South China Normal University, Guangzhou 510631
Yonghong Hu：Department of Medical Oncology, Cancer Center, Sun Yat-Sen University of Medical Sciences, Guangzhou 510060
备注：This work was supported by the Natural Science Foundation of Guangdong Province (No. 021093), Keystone Science andTechnology Foundation of Guangdong Province (No. 2KM04502S) and Natural Science Scientific Research Foundation of Guangdong Universities (No. 200018). Y. Meng is the author to whom the correspondence should be addressed, his e-mail address is firstname.lastname@example.org
【1】J. C. Martin, R. M. Wartell, and D. C. O'Shea, Proc. Natl. Acad. Sci. USA 75, 5483 (1978).
【2】J. Mazerski and K. Muchewicz, Acta. Biochimica Polonica 47, 65 (2000).
【3】T. Kursar and G. Holzwarth, Biochemistry 15, 3352 (1976).
【4】V. V. Andrushchenko, S. V. Kornilova, L. E. Kapinos, E. V Hackl, V. L. Galkin, D. N. Grigoriev, and Y. P. Blagoi, J. Mol. Struct. 408, 225 (1997).
【5】E. V. Hackl, S. V. Kornilova, L. E. Kapinos, V. V. Andrushchenko, V. L. Galkin, D. N. Grigoriev, and Y. P. Blagoi, J. Mol. Struct. 408, 229 (1997).
【6】X. Z. Feng, L. Zhang, C. Wang, and C. L. Bai, Science in China (Series C) (in Chinese) 29, 39 (1999).
【7】Y. Y. Meng and S. H. Liu, Acta Photon. Sin. (in Chinese) 30, 794 (2001).
【8】Y. Y. Meng and S. H. Liu, Spectroscopy and Spectral Analyse (in Chinese) 20, 280 (2000).
【9】S. H. Liu and Y. Y. Meng, Acta Opt. Sin. (in Chinese) 20, 529 (2000).
【10】H. Deng, V. A. Bloomfield, J. M. Benevides, and G. J. Jr. Thomas, Biopolymers 50, 656 (1999).
【11】J. G. Duguid, V. A. Bloomfield, J. M. Benevides, and G. J. Jr. Thomas, Biophys. J. 69, 2623 (1995).
【12】J. G. Duguid, V. A. Bloomfield, J. M. Benevides, and G. J. Jr. Thomas, Biophys. J. 65, 1916 (1993).
【13】Marclanglais, H. A. Tajmir-Riahi, and R. Savoie, Biopolymers 30, 743 (1990).
【14】B. Prescott, W. Steinmetz, and G. J. Jr. Thomas, Biopolymers 23, 235 (1984).
【15】G. J. Jr. Thomas, J. M. Benevides, S. A. Overman, T. Ueda, K. Ushizawa, M. Saitoh, and M. Tsuboi, Biophys. J. 68, 1073 (1995).
【16】J. M. Benevides, A. H.-J. Wang, G. A. van der Marel, J. H. van Boom, and G. J. Jr. Thomas, Biochemistry 27, 931 (1988).
【17】J. M. Benevides, A. H.-J. Wang, A. Rich, Y. Kyogoku, G. A. van der Marel, J. H. van Boom, and G. J. Thomas, Biochemistry 25, 41 (1986).
【18】K. L. Aubrey, S. R. Casjens, and G. J. Jr. Thomas, Biochemistry 31, 11835 (1992).
【19】J. G. Duguid, V. A. Bloomfield, J. M. Benevides, and G. J. Thomas, Biophys. J. 71, 3350 (1996).
【20】A. Toyama, H. Takeuchi, and I. Harada, J. Mol. Struct. 242, 87 (1991).
【21】R. M. Wartell and J. T. Harrell, Biochemistry 25, 2664 (1986).
【22】E. H. S. Anwander, M. M. Probst, and B. M. Rode, Inorg. Chim. Acta 137, 203 (1987).
Guiqiang Liu, Yaoyong Meng, Songhao Liu, Yonghong Hu, "Raman spectroscopic studies on the conformational changes of calf thymus DNA induced by Cd2+ ions," Chinese Optics Letters 2(3), 154-156 (2004)