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Raman spectroscopic studies on the conformational changes of calf thymus DNA induced by Cd2+ ions

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Abstract

We used Raman spectroscopy to study the conformational changes of DNA induced by Cd2+ ions in different Cd2+ concentrations solution. The experimental results show that when the Cd^(2+)/PO^(-)_(2) ratio R increased from 0 to 3.0, the band 835.0 cm^(-1) shifted about 8 cm^(-1), and the overlapping spectra of 1446.0 and 1461.0 cm^(-1) separated and moved to 1441.0 and 1458.0 cm^(-1), respectively. This indicates that the conformation of DNA has changed from a "normal" B-form to a "modified" B'-form. At the same time, changes of other bands demonstrate that parts of base stacking collapse and some hydrogen bonds between AT are disrupted, AT base pairs are damaged more larger than GC base pairs.

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所属栏目:medical optics and biotechnology

收稿日期:2003-11-11

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Guiqiang Liu:Institute of Laser Life Sciences, South China Normal University, Guangzhou 510631
Yaoyong Meng:Institute of Laser Life Sciences, South China Normal University, Guangzhou 510631
Songhao Liu:Institute of Laser Life Sciences, South China Normal University, Guangzhou 510631
Yonghong Hu:Department of Medical Oncology, Cancer Center, Sun Yat-Sen University of Medical Sciences, Guangzhou 510060

联系人作者:Yaoyong Meng(mengyy@scnu.edu.cn)

备注:This work was supported by the Natural Science Foundation of Guangdong Province (No. 021093), Keystone Science andTechnology Foundation of Guangdong Province (No. 2KM04502S) and Natural Science Scientific Research Foundation of Guangdong Universities (No. 200018). Y. Meng is the author to whom the correspondence should be addressed, his e-mail address is mengyy@scnu.edu.cn

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引用该论文

Guiqiang Liu, Yaoyong Meng, Songhao Liu, Yonghong Hu, "Raman spectroscopic studies on the conformational changes of calf thymus DNA induced by Cd2+ ions," Chinese Optics Letters 2(3), 154-156 (2004)

被引情况

【1】廖昱博,孟耀勇,雷浩东,王英. A·C碱基错配对的喇曼光谱研究. Chinese Optics Letters, 2008, 6(1): 61-63

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