Chinese Optics Letters, 2004, 2 (3): 03162, Published Online: Jun. 6, 2006
Cross-talk correction in dual-labeled fluorescent microarray scanning Download: 577次
170.6280 spectroscopy fluorescence and luminescence 180.2520 fluorescence microscopy 260.2510 fluorescence
Abstract
Cross-talk phenomenon in dual-labeled fluorescent microarray scanning is analyzed from cross-excitation and cross-emission. It is turned out that the spectral overlap of the fluorophores is crucial for cross-talk error, and this error can be corrected by an image subtraction method. The experiment was successfully applied to separate the Cy3 channel and the Cy5 channel in microarray scanning. The cross-talk error was reduced from more than 1% to about 0.1%.
Liqiang Wang, Zukang Lu, Xuxiang Ni. Cross-talk correction in dual-labeled fluorescent microarray scanning[J]. Chinese Optics Letters, 2004, 2(3): 03162.