激光生物学报, 2004, 13 (3): 224, 网络出版: 2006-06-11
细胞内微丝骨架的变化对人肝癌Bel-7402细胞形态的影响
The Effect of Change in Cellular Microfilament Cytoskeleton on Cellular Morphology of Human Bel-7402 Hepatocarcinoma Cell
激光扫描共聚焦显微镜 人肝癌Bel-7402细胞 F-肌动蛋白 微丝骨架 细胞松弛素-B confocal scanning laser microscope （CSLM） human Bel-7402 hepatocarcinomacell F-actin microfilament cytoskeleton cytochalasin-B（Cyt-B）
摘要
癌细胞的形态及癌细胞的生物学行为(如粘附、迁移和浸润)与细胞内骨架系统密切相关, 本研究利用Phalloidin-FITC标记人肝癌Bel-7402细胞内F-肌动蛋白(F-actin), 使用激光扫描共聚焦显微镜观测细胞微丝骨架与癌细胞形态的关系, 及Cyt-B处理Bel-7402细胞后,癌细胞内微丝骨架的变化.结果显示:癌细胞内F-actin解聚,F-actin小体的聚集重组是癌细胞的形态变化的主要因素之一.
Abstract
Cellular morphology and cellular biological activities (i,e,. adhesion, mobility and invasion) are related to intracellular cytoskeleton. In the present study we used Phalloidin-FITC to label cellular F-actin of human Bel-7402 hepatocarcinoma cell. Intracellular microfilament of Bel-7402 cell and the change of cancer cellular microfilament cytoskeleton after Bel-7402 cell being treated with Cyt-B was observed by confocal scanning laser microscope (CSLM). The results showed that the morphological change of ...
林珏龙, 陈耀文, 李璟, 方昆阳, 许险峰, 霍霞. 细胞内微丝骨架的变化对人肝癌Bel-7402细胞形态的影响[J]. 激光生物学报, 2004, 13(3): 224. 林珏龙, 陈耀文, 李璟, 方昆阳, 许险峰, 霍霞. The Effect of Change in Cellular Microfilament Cytoskeleton on Cellular Morphology of Human Bel-7402 Hepatocarcinoma Cell[J]. Acta Laser Biology Sinica, 2004, 13(3): 224.
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