Chinese Optics Letters, 2006, 4 (6): 348, Published Online: Jun. 12, 2006
Steady state and time-resolved autofluorescence studies of human colonic tissues Download: 510次
170.6280 Spectroscopy, fluorescence and luminescence 170.6510 Spectroscopy, tissue diagnostics 300.0300 Spectroscopy
Abstract
Steady state and time-resolved autofluorescence spectroscopies are employed to study the autofluorescence characteristics of human colonic tissues in vitro. The excitation wavelength varies from 260 to 540 nm, and the corresponding fluorescence emission spectra are acquired from 280 to 800 nm. Significant difference in fluorescence intensity of excitation-emission matrices (EEMs) is observed between normal and tumor colonic tissues. Compared with normal colonic tissue, low nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD), and high amino acids and protoporphyrin IX (PpIX) fluorescences characterize high-grade malignant tissue. Moreover, the autofluorescence lifetimes of normal and carcinomatous colonic tissues at 635 nm under 397-nm excitation are about 4.32+-0.12 and 18.45+-0.05 ns, respectively. The high accumulation of endogenous PpIX in colonic cancers is demonstrated in both steady state and time-resolved autofluorescence spectroscopies.
Buhong Li, Zhenxi Zhang, Shusen Xie. Steady state and time-resolved autofluorescence studies of human colonic tissues[J]. Chinese Optics Letters, 2006, 4(6): 348.