光学学报, 1999, 19 (4): 567, 网络出版: 2006-08-09
荧光相位成像的光束扫描法
Fluorescence Phase-Sensitive Imaging with Beam Scanning
摘要
利用计算机控制振镜进行光束扫描, 用锁相放大器进行增益调制与相位调节, 最后再用计算机重建图像, 实现钛宝石和红宝石的相位成像。 分别完成了激发光和荧光相移为π/12、 π/4和7π/12的成像, 分析了系统的时间分辨率和空间分辨率, 提出了实用化发展的方向。
Abstract
Employing computer to control optical scanners and lock-in amplifier to modulate gain and shift phase, a fluorescence phase-sensitive imaging system has been investigated for ruby and Ti-sapphire experimentally. The images with phase shift of π/12, π/4, and 7π/12 were obtained. The time resolution and the space resolution, as well as the practicality, of the system are analyzed.
丁刚, 景春阳, 肖玲, 蒋建华, 张桂燕, 林福成. 荧光相位成像的光束扫描法[J]. 光学学报, 1999, 19(4): 567. 丁刚, 景春阳, 肖玲, 蒋建华, 张桂燕, 林福成. Fluorescence Phase-Sensitive Imaging with Beam Scanning[J]. Acta Optica Sinica, 1999, 19(4): 567.