通过激光微束穿刺法，将β-1,3-葡聚糖酶及几丁质酶基因双价植物表达载体pBLGC导入棉花幼胚。转化一代棉花幼苗用蘸根法进行抗黄萎病筛选，将存活的苗移入病圃进行了卡那霉素(Kan 1%)抗性测定。结果表明，在移栽的29株幼苗中，有9株表现出明显的Kan抗性，对9株抗Kan植株进行了PCR检测，结果显示，其中7株表现为阳性。病圃中的T1代转基因植株在经历了黄萎病发病高峰期后，7株PCR阳性植株表现明显抗病，并已正常开花结铃，其他T1代植株及对照植株全部因后期发病死亡。这初步证明外源基因已整合到棉花基因组中，且使转基因植株对黄萎病表现出一定的抗性。

By the method of laser microbeam puncture, the maturing embryoes of cotton were transformed with plant bivalent expression vector pBLGC including β-1,3-glucanase and chitinase genes. Fungal challenge of T1 generation with Verticillium albo-atrum were carried out by dipping their roots into hyphal and spores solution. The survived T1 seedings were subjected to Kan-resistant assay, and the results showed that 9 out of 29 T1 plants showed obvious Kanamycin resistance (Kan 1%). Upon PCR analysis, 7 out of 9 Kan-resistant plants produced a PCR fragment with expected size, indicating that they are probably transgenic. After T1 plants in disease-inducing plot were subjected to the period, in which the Veritcillium wilt serious took place, 7 PCR positive plants showed obvious resistance to this fungus, at last, flowered and bore cotton bolls normally, while the other T1 plants and the non-transformants all died of infection. This result primarily suggested that the foreign genes had integreted in cotton genome, and the transgenic plants showed high resistance to Verticillium wilt.