用532 nm的激光作为激发光源,分别测量正常人血液及血液组分(血浆、血小板、红细胞)的荧光光谱。结果显示,全血在 630 nm及710 nm附近出现荧光峰值,其各组分的荧光光谱有明显差异,其中血浆的荧光光谱可作为临床诊断依据。另外,比较正常人及乙型肝炎患者血浆标本的荧光光谱发现,其 738 nm处的峰值强度有显著差异。

A 532 nm laser was used as exciting source to measure the fluorescence spectra of human blood and its components (red blood cell, blood platelet and blood plasma). The spectra of blood have peaks near 630 nm and 710 nm, and the spectra of blood components are obviously different from them. The spectrum of plasma has a clear peak at 738 nm. Contrary to the normal blood plasma, the plasma spectrum of HBsAg positive blood has a very strong peak at 738 nm,which can be used for diagnosis of hepatitis B.