Chinese Optics Letters, 2006, 4 (8): 464, Published Online: Aug. 31, 2006  

Research on optical biosensor with up-converting phosphor marker

Author Affiliations
1 Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800
2 Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071
3 Chinese Academy of Inspection and Quarantine, Beijing 100025
Abstract
An optical biosensor with up-converting phosphor (UCP) marker is developed for the sensitive rapid immunoassay to the specific biomolecule. UCP can emit visible light when excited by infrared light. Through detecting and analyzing the content of UCP particles on the test strip after immunoreaction, the concentration of target analyte in the sample can be obtained. The detection sensitivity to plague IgG is better than 5 ng/ml; to plague FI-Ab is better than 100 pg/ml; to plague Yersinia pestis cell is better than 3*10^(4) CFU/ml. Good linear response characteristics and an excellent correlation (R2>=0.95) have been verified by quantitative detection results. In the practical application, detection results to 167 analytic samples have an excellent consistency with those obtained by reverse hemagglutination test. The up-converting phosphor technology (UPT) based biosensor has stable, reliable, and sensitive performances. It can meet the need of various bioassay applications.

Yongkai Zhao, Lei Zhou, Jing Wang, Lihua Huang, Zhongqiang Yan, Huijie Huang, Ruifu Yang, Lei Liu, Bingqiang Ren, Xiangzhao Wang. Research on optical biosensor with up-converting phosphor marker[J]. Chinese Optics Letters, 2006, 4(8): 464.

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!