首页 > 论文 > 光子学报 > 35卷 > 4期(pp:555-560)

上转换发光免疫试纸条扫描检测系统研究

Scanning Detection System of UPT-Based Immunoassay Test Strip

  • 摘要
  • 论文信息
  • 参考文献
  • 被引情况
  • PDF全文
分享:

摘要

研制了一种以上转换发光材料作为标记物的扫描检测系统,有别于传统的测量方法,可做出定量和多重分析.扫描检测系统以扫描方式逐点测量经生物反应而结合上去的UCP颗粒的含量,记录整个免疫试纸条的信息,计算出被测样品中特定生物分子的浓度.实验结果表明,该系统对兔抗鼠疫免疫球蛋白(IgG)标准样品的检测灵敏度达到百ng/mL量级,且具有变异系数小于6%的重复性,符合生物免疫测定的要求.

Abstract

A new scanning system is developed,which is a new biosensor for the immunoassay by using the label of up-converting phosphors (UCP),which is different from traditional methods and suitable for multiplexing and quantitative analysis. The system scans the strip point by point in order to record all the information on it,then figures out the concentration of the biological molecular from the content of the UCP on the test strip. It is effective to take precise detection of micro-immunoreaction to the degree of hundreds ng/ml with fine stability,which meet the requirements of the immunoassay.

广告组1 - 空间光调制器+DMD
补充资料

中图分类号:Q631

所属栏目:光生物学

基金项目:国家自然科学基金(60378024)资助

收稿日期:2005-01-20

修改稿日期:--

网络出版日期:0001-01-01

作者单位    点击查看

卢健:中国科学院上海光学精密机械研究所,上海 201800中国科学院研究生院,北京 100039
周蕾:军事医学科学院微生物流行病研究所,北京 100071
赵永凯:中国科学院上海光学精密机械研究所,上海 201800中国科学院研究生院,北京 100039
黄立华:中国科学院上海光学精密机械研究所,上海 201800中国科学院研究生院,北京 100039
刘蕾:中国科学院上海光学精密机械研究所,上海 201800中国科学院研究生院,北京 100039
杨瑞馥:军事医学科学院微生物流行病研究所,北京 100071
黄惠杰:中国科学院上海光学精密机械研究所,上海 201800中国科学院研究生院,北京 100039

联系人作者:卢健(lujian_1980@163.com)

【1】Zarling D A,Rossi M J,Reppers N A,et al. Upconverting reporters for biological and other assays using laser excitation techniques. US Patent,C08g,6537829,2003~03~25

【2】Niedbala R S,Feindt H,Kardos K,et al. Detection of analytes by immunoassay using up-converting phosphor technology. Analytical Biochemistry,2001,293(l):22~30

【3】Niedbala R S,Vail T L,Fiendt H,et al. Multiphoton upconverting phosphors for use in rapid immunoassays.SPIE,2000,3913: 193~203

【4】冯鸣,阮双琛,杜晨林,等.掺Yb3++双包层光纤激光器中频率上转换产生可见光的实验研究.光子学报,2004,33(6):648~650
Fen M,Nuan S S,Du C L,et al. Acta Photonica Sinica,2004,33(7): 814~817

【5】Qin G S,Qin W P,Huang S H,et al. Up-converting Yb3+-Er3+ co-doped amorphous fluoride thin films prepared by pulsed-laser deposition for visible light source. Solid State Communications,2001,120(5) :211~244

【6】杨玲珍,董淑福,郑瑶雷,等.掺Yb3+双包层光纤中的绿光荧光分析.光子学报,2003,32(8):897~899
Yang L Z,Dong S F,Zhen Y L,et al. Acta Photonica Sinica,2003,32(8): 897~899

【7】Shang L,Fiendt H,Giannaras G,et al. Preparation,characterization,and fabrication of uniform coated Y2O2S ∶RE3+ up-converting phosphors particles for biological detection application. SPIE,2002,4809: 100~109

【8】Hampl J,Hall M,Mufti N,et al. Up-converting phosphor reporters in immunochromatographic assays. Analytical Biochemistry,2001,288(2):176~187

【9】Corstjens P,Zuiderwijk M,Brink A,et al. Use of upconverting phosphor reporters in lateral-flow assays to detect specific nucleic acid sequences: a rapid,sensitive DNA test to identify human papillomavirus type 16 infection. Clinical Chemistry,2001,47 (10) : 1885~1893

【10】Zijmans H,Bonnet J,Burton J,et al. Detection of cell and tissue surface antigens using up-converting phosphors: a new technology. Analytical Biochemistry,1999,267(1): 30~36

【11】周望,陈林森,叶燕.共焦光学传感头用于红外上转换油墨的微弱光检测.光子学报,2004,33(7):814~817
Zhou W,Chen L S,Ye Y. Acta Photonica Sinica,2004,33(7): 814~817

引用该论文

Lu Jian,Zhou Lei,Zhao Yongkai,Huang Lihua,Liu Lei,Yang Ruifu,Huang Huijie. Scanning Detection System of UPT-Based Immunoassay Test Strip[J]. ACTA PHOTONICA SINICA, 2006, 35(4): 0555

卢健,周蕾,赵永凯,黄立华,刘蕾,杨瑞馥,黄惠杰. 上转换发光免疫试纸条扫描检测系统研究[J]. 光子学报, 2006, 35(4): 0555

被引情况

【1】谢承科,张友宝,黄立华,屈建峰,郭小娴,黄惠杰. 上转换磷光生物传感器信号处理算法. 光子学报, 2009, 38(12): 3256-3260

【2】董国亚,赵 翔,张 燕,孙传强. 便携式上转换荧光试纸条检测仪的研制. 光学 精密工程, 2017, 25(3): 584-590

您的浏览器不支持PDF插件,请使用最新的(Chrome/Fire Fox等)浏览器.或者您还可以点击此处下载该论文PDF