强化生物除磷是废水生物除磷的主要技术手段。 在厌氧与好氧交替运行的反应过程中, 污泥中聚β羟基烷酸酯（PHA）经历了厌氧储存和好氧降解的过程, 其位于1 740 cm-1吸收峰亦呈现出先增强后减弱的变化。 通过比较PHA标准品的红外光谱图, 实现了活性污泥PHA的红外特征归属, 1 740 cm-1红外吸收峰源于PHA羰基特征吸收。 利用高斯分峰法将相互重叠的PHA峰、 蛋白质Ⅰ峰和蛋白质Ⅱ峰进行了分离, PHA与蛋白质Ⅰ峰的峰面积比值与PHA测定值有较好相关性, 相关系数可达0.873, 峰面积比值可反映污泥中PHA的变化趋势。 选取1 480~1 780 cm-1区域红外光谱, 经过归一化处理并转化为吸光度数据后, 结合污泥样品PHA含量的色谱分析结果, 应用偏最小二乘法建立了污泥样品红外光谱与PHA含量的关系模型, 模型预测值与测量值具有较好的一致性, 结合未知浓度活性污泥样品的红外光谱以及该关系模型, 可以迅速预测未知污泥样品中的PHA含量。 该方法的研究为污泥胞内PHA的快速表征和定量分析提供了新的分析方法。

Enhanced biological phosphorus removal (EBPR) is the main phosphorus removal technique for wastewater treatment. During the anaerobic-aerobic alternative process, the activated sludge experienced the anaerobic storage of polyhydroxy-β-alkonates (PHA) and aerobic degradation, corresponding the infrared peak intensity of sludge at 1 740 cm-1 increased in the aerobic phase and declined in the anaerobic phase. Compared with PHA standard, this peak was indentified to attribute the carbonyl of PHA. The overlapping peaks of PHA, protein Ⅰ and Ⅱ bands were separated using Gaussian peak fitting method. The infrared peak area ratios of PHA versus protein I had a good relationship with the PHA contents measured by gas chromatography, and the correlation coefficient was 0.873. Thus, the ratio of the peak area of PHA versus protein Ⅰ can be considered as the indicator of the PHA content in the sludge. The infrared spectra of 1 480~1 780 cm-1 was selected, normalized and transferred to the absorption data. Combined with the chromatography analysis of PHA content in the sludge sample, a model between the Fourier-transform infrared spectroscopy (FTIR) spectra of the sludge and PHA content was established, which could be used for the prediction of the PHA content in the unknown sample. The PHA content in the sludge sample could be acquired by the infrared spectra of the sludge sample and the established model, and the values fitted well with the results obtained from chromatograph. The results would provide a novel analysis method for the rapid characterization and quantitative determination of the intracellular PHA content in the activated sludge.