采用红外光谱并结合二维相关红外光谱法对生三七和熟三七进行了研究, 分析比较三七炮制前后化学成分的变化。 在一维红外谱图上, 生三七和熟三七在1 200～400 cm-1波段内的相似度较高, 不同的是熟三七在2 925, 2 855, 1 746, 1 460, 1 376, 1 158 cm-1出现了花生油的特征峰, 这是由于采用煎炸法炮制三七所残留的花生油所引起的。 两者药效组分的特征差异在二维相关红外谱图上显示得更为明显和直观, 在1 400～1 700 cm-1区域, 生三七仅在1 650 cm-1附近有一个强自动峰, 而熟三七在1 469和1 640 cm-1附近均有自动峰。 在1 120～1 250 cm-1区域, 生三七和熟三七共有1 139(1 137), 1 194(1 196), 1 219(1 221)cm-1三个自动峰, 不同的是各自动峰的相对强度发生了变化, 生三七经炮制后1 139 cm-1峰的增强, 而1 194 cm-1峰减弱。 二维相关红外光谱的变化规律说明了三七药材在炮制的过程中发生的主要变化是黄酮、 糖类、 皂苷等成分的分解。 该方法揭示了三七在炮制过程中所发生的物理化学变化过程, 从红外光谱的角度解释了三七“生消熟补”的药理。

The chemical differences of panax notoginseng before and after processing were analyzed by Fourier transform Infrared spectroscopy (FTIR) combined with two-dimensionalcorrelation spectroscopy (2D-IR). Compared with conventional IR spectra of the samples, the FTIR spectra of panax notoginseng and its processed products were similar in the range of 1 200～400 cm-1. The difference was that prepared panax notoginseng had strong and characteristic peaks at 2 925, 2 855, 1 746, 1 460, 1 376 and 1 158 cm-1, which all arose from the characteristic vibration of peanut oil. This was because there was some peanut oil left in the panax notoginseng, when panax notoginseng after processing. Obvious differences were observed between 2D-IR spectra of them, in the range of 1 400～1 700 cm-1, there was only one auto peaks at 1 650 cm-1 in the spectra of panax notoginseng, but there were auto peaks at 1 469 and 1 640 cm-1 in the spectra of prepared panax notoginseng. In the range of 1 400～1 700 cm-1, the 2D-IR spectra of panax notoginseng and its processed product present characterstic peaks at 1 139(1 137), 1 194(1 196), 1 121(1 221)cm-1 respectively, but the relative intensities of auto peaks were changed. For example, auto peak around 1 139 cm-1 was enhanced, but auto peak around 1 194 cm-1 was weakened. The results of 2D-IR correlation spectroscopy indicated the decomposition of flavonoids, saccharides and saponins. This method can track dynamically the processingprocedure of panax notoginseng and reveal the main tansformations, so it can explain the pharmacology of panax notoginseng and its processed product by FTIR and 2D-IR.