Chinese Optics Letters, 2008, 6 (1): 0161, Published Online: Jan. 29, 2008  

Characterization of PolyA and PolyC mismatches by Raman spectroscopy

Author Affiliations
Photonic Chinese Medicine Lab, South China Normal University, Guangzhou 5106312School of Physics and Electronic Information Science, Gannan Teachers College, Ganzhou 3410003Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510631
Abstract
A·C mismatches are studied by Raman spectral characterization of PolyA, PolyC, and their equimolar complex in solution of 0.14 mol/L Na+, pH7.0. Experimental results show that A·C mismatches occur to be A/B (mainly A) conformers, and unlike Watson-Crick base pairing, this kind of mismatches is stabilized by only one hydrogen bond involving cytosine N4H2 and adenine N7. The formation of A·C complex makes the base stacking interactions much stronger, and conformation of the backbone more ordered, which leads to obvious Raman hypochromic effect with some shifts in corresponding bands.

Yubo Liao, Yaoyong Meng, Haodong Lei, Ying Wang. Characterization of PolyA and PolyC mismatches by Raman spectroscopy[J]. Chinese Optics Letters, 2008, 6(1): 0161.

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