Chinese Optics Letters, 2008, 6 (1): 0161, Published Online: Jan. 29, 2008
Characterization of PolyA and PolyC mismatches by Raman spectroscopy
A·C碱基错配对 喇曼光谱 多聚腺嘌呤核苷酸 多聚胞嘧啶核苷酸 170.5660 Raman spectroscopy 300.6170 Spectra 300.6330 Spectroscopy, inelastic scattering including Raman 300.6450 Spectroscopy, Raman 300.6390 Spectroscopy, molecular
Abstract
A·C mismatches are studied by Raman spectral characterization of PolyA, PolyC, and their equimolar complex in solution of 0.14 mol/L Na+, pH7.0. Experimental results show that A·C mismatches occur to be A/B (mainly A) conformers, and unlike Watson-Crick base pairing, this kind of mismatches is stabilized by only one hydrogen bond involving cytosine N4H2 and adenine N7. The formation of A·C complex makes the base stacking interactions much stronger, and conformation of the backbone more ordered, which leads to obvious Raman hypochromic effect with some shifts in corresponding bands.
Yubo Liao, Yaoyong Meng, Haodong Lei, Ying Wang. Characterization of PolyA and PolyC mismatches by Raman spectroscopy[J]. Chinese Optics Letters, 2008, 6(1): 0161.