应用激光击穿光谱法(LIBS)探测了体液中各种物质的含量。脉冲Nd:YAG激光器产生的波长1064 nm的激光束(脉宽10 ns,重复频率10 Hz,激光能量约300 mJ)经凸透镜聚焦后,击穿模拟体液(质量分数为10%葡萄糖和0.9%NaCl的混合水溶液)产生激光等离子体,利用中阶梯光谱仪和像增强CCD(ICCD)探测其光谱信号,开展相应的激光击穿光谱研究。实验结果表明应用激光击穿光谱的技术完全可以同时检测出溶液中的有机物(葡萄糖)和金属离子,而且金属离子的检测灵敏度明显优于有机物,各待测物质的特征谱线强度与其含量存在指数关系。该方法为体液中微量元素的精确测量提供了实验依据。

A new method of detecting body fluid is brought forward by studying the laser-induced spectroscopy (LIBS). The sample solution, which is comprised of 10% glucose and 0.9% NaCl, is broken-down by focusing the 1. 064 μm Nd:YAG laser beam (energy ～300 mJ, frequency 10 Hz, pulse width ～10 ns). And the plasmas spectra are collected by an experimental setup including echelle spectrograph and ICCD. The experimental results show that the organic matter ( as glucose) and metal elements can be synchronously analyzed by LIBS and the metal elements are more sensitive than the organic matter. The results also indicate that there is the exponential relation between the intensities of characteristic spectra and concentration. These results provide some experimental evidences for extending this mothed to be a new way of accurately detecting trace element in body fluid.