Chinese Optics Letters, 2008, 6 (12): 879, Published Online: Dec. 17, 2008
Two-photon imaging of lymphoma cells targeted by gold nanoparticles Download: 665次
170.3880 Medical and biological imaging 170.4580 Optical diagnostics for medicine 300.6410 Spectroscopy, multiphoton
Abstract
Gold nanoparticles (NPs) have highly efficient multi-photon-induced luminescence. In this paper, we record the two-photon images of gold NPs, lymphoma cell line Karpas 299, and Karpas 299 incubated with 30-nm-diameter gold NPs and ACT-1 antibody conjugates (Au30-ACT-1 conjugates) by using a multi-photon microscopy system. Due to the specific conjugation of ACT-1 antibody and cell membrane receptor CD25, gold NPs are only bound to the surface of cell membrane of Karpas 299. The luminescence intensity of gold NPs is higher than that of cells at 750-nm laser excitation. By comparing the images of Karpas 299 cells incubated with and without gold NPs, it is found that by means of gold NPs, we can get clear cell images with lower excitation power. Their excellent optical and chemical properties make gold NPs an attractive contrast agent for cellular imaging.
Xiaochao Qu, Jing Wang, Cuiping Yao, Zhenxi Zhang. Two-photon imaging of lymphoma cells targeted by gold nanoparticles[J]. Chinese Optics Letters, 2008, 6(12): 879.