基于棉涤线的毛细作用, 构建成“Y”型微流控分析通道, 自行研制了一种可调控试样流速的微流控分析通道装置, 实现了待测液和显色剂同时进样, 研究建立了一种“Y”型棉涤线微流控分析通道分析测定新方法。进行了分光光光度法与Scan-Adobe Photoshop软件处理两种检测方法的比较, 结果表明光度法检出限低; Scan-Adobe Photoshop软件处理法操作简捷, 分析速度快, 样品用量少。应用于亚硝酸根的分析测定, 两种检测方法的线性范围和检出限分别为1.0~70 μmol·L-1, 0.66 μmol·L-1(光度法); 50~450和45.10 μmol·L-1(Scan-Adobe Photoshop软件处理法)。回收率在96.7%~104.0%之间。该微流控通道分析方法成本低廉, 分析速度快, 对土壤和水样中亚硝酸根进行分析测定, 结果满意。

A novel method based on Y-shaped microfluidic channel was firstly proposed in this study. The microfluidic channel was made of two cotton-polyester threads based on the capillary effect of cotton-polyester threads for the determination solutions. A special device was developed to fix the Y-shaped microfluidic channel by ourselves, through which the length and the tilt angle of the channel can be adjusted as requested. The spectrophotometry was compared with Scan-Adobe Photoshop software processing method. The former had a lower detection limit while the latter showed advantages in both convenience and fast operations and lower amount of samples. The proposed method was applied to the determination of nitrite. The linear ranges and detection limits are 1.0~70 μmol·L-1, 0.66 μmol·L-1 (spectrophotometry) and 50~450 μmol·L-1, 45.10 μmol·L-1 (Scan-Adobe Photoshop software processing method) respectively. This method has been successfully used to the determination of nitrite in soil samples and moat water with recoveries between 96.7% and 104%. It was proved that the proposed method was a low-cost, rapid and convenient analytical method with extensive application prospect.