光学学报, 2008, 28 (s2): 143, 网络出版: 2009-01-05
细胞低电压破碎及其效果的光学检测方法
Crushing Cells under Low-Voltage and Its Optical Testing Method
光电子学 细胞破碎 微流控芯片 低电压 光学检测 optoelectronics crushing cells microfluidic chip low voltage optical testing
摘要
为获取细胞的内容物(蛋白质, DNA, RNA等), 探讨光电子技术在细胞破碎方面的应用, 理论上引入了BHK21(幼仓鼠肾细胞)的初始跨膜电位, 对BHK21细胞跨膜电压模型进行修正。设计了细胞破碎的控制及检测系统。制作的细胞破碎芯片电极的宽度和间距均为50 μm, 每个电极的长度为0.4 cm, 电极厚度为0.5 μm。搭建了用于细胞破碎、控制及自动检测的检测系统, 系统中以倒置显微镜作为主要光路部件, 采用300万像素的CCD摄像头采集图像信息, 并基于显微数字图像处理软件实现细胞图像处理。实验表明, BHK21细胞破碎的最佳电压幅度为3 V, 脉冲宽度为3 ms, 所搭建的光学检测系统能够自动识别BHK21数字图像, 并能对在显微视窗中的BHK21细胞实现自动计数等功能。
Abstract
It is intended to obtain the cell contents (protein, DNA, RNA etc.) and probe into the application of optoelectronic technology for crushing cell. In theory BHK21 initial transmembrane voltage is introduced to modify the voltage model. Control and detecting systems for crushing cells are made as follows: crushing cells chip electrodes 50 μm in both width and pitch interval, with a length of 0.4 cm and thickness of 0.5 μm each. The system could be used in cells crushing, controlling and automatic testing. It takes inverted microscope as the main light path system, collects pictures with 3 mega pixel CCD camera and deals them with processing software of micro-digital image. The experiment shows that this optical testing system could automatically identify and count BHK21 in micro-windows. For crushing BHK21 cells, the most suitable voltage range and pulse width are 3 V and 3 ms.
陈建军, 廖红华, 王骏, 韩鹤友, 于军. 细胞低电压破碎及其效果的光学检测方法[J]. 光学学报, 2008, 28(s2): 143. Chen Jianjun, Liao Honghua, Wang Jun, Han Heyou, Yu Jun. Crushing Cells under Low-Voltage and Its Optical Testing Method[J]. Acta Optica Sinica, 2008, 28(s2): 143.