为能够充分利用细胞光散射的全息信息, 以更好地对生物细胞类别进行解构, 根据血液细胞的光穿透特性, 建立了血液细胞中红细胞(RBC)、白细胞(WBC)中粒细胞和非粒细胞的典型细胞光散射模型。借助于数字相位显微(DPM)技术的基本方法和实验条件参数, 应用VirtualLabTM光学虚拟仿真实验系统, 获得了血液细胞在光散射下的虚拟仿真相位分布和光强分布。针对所获得的相位和光强分布图谱进行了分布特征分析, 发现了相位分布直接与细胞形态结构密切相关。该点可作为细胞类别分检的一个新的重要依据, 该方法相对于3D重建方法来说计算量少、无需很多的边界条件。这种由细胞相位分布解构细胞形态的检测方法可为提升细胞检测技术和发展检测方法的研究提供一种新突破方向。

In order to measure different life-cells with more scattering information of hologram, light propagation models of red blood cell (RBC) and white blood cell (WBC) are built based on their optical characters and the digit holograph function of the VirtualLabTM imitation system. The distributions of cells, scattering phase and their intensity are achieved after using this virtual imitation experiment system according to the digit phase microscopy (DPM) method and its experimental parameters. These distribution characters are studied and it is found that the phase distributions can be used as an important criterion for testing cells, distribution because of their relationship. Comparing the 3-D building method, this cell-constructing method based on the phase analysis needs fewer confine condition and less counting. It provides a breakthrough for developing cell’s measurement in technique and method.