作为一种非线性光学成像手段, 多光子激发已经在生物医学影像学域发挥了重要作用。虽然更短的脉冲可以激发更强的非线性效应, 但由于高阶色散的存在, 目前国际上的多光子激发成像工作一直采用大于100 fs的亚皮秒脉冲激发。通过多光子脉冲间干涉相位扫描法, 成功地将10 fs脉冲引入到多光子生物成像领域。通过补偿高阶色散后, 荧光信号得到了显著增强, 探测深度明显增加, 且可使多种染料同时被激发。

As an important nonlinear optical imaging method, multiphoton excitation has demonstrated its wide application in biomedical imaging. Although shorter pulse can induce a higher nonlinear effect, most applications are still restricted in 100 fs region due to the existence of high-order dispersion. Through the compensation of high-order dispersion with multiphoton intrapulse interference, we successfully introduce 10 fs pulse into multiphoton biomedical imaging. We find that a deeper penetration and a correct excitation of multiple dyes can be obtained with this method.