中国激光, 2009, 36 (10): 2625, 网络出版: 2009-10-23
脉冲激光沉积类金刚石膜和细胞相容性研究
Experiment of Diamond-Like Carbon Film Deposited by Pulsed Laser and Cell Compatibility of Diamond-Like Carbon Film
激光技术 脉冲激光沉积法 类金刚石膜 机械瓣膜 黏附性 细胞相容性 laser technique pulsed laser deposition diamond like carbon film mechanical heart valve adhesion cell compatibility
摘要
中国科学院“计划”和合肥工业大学博士基金(GDBJ2008043)资助项目。
Abstract
The experiment was to avoid cruor on the surface of the mechanical heart valve. Firstly diamond-like carbon (DLC) films were deposited on the mechanical heart valve,Al2O3,316 L stainless steel and silicon substrates by the pulsed laser deposition (PLD) at same experiment condition. The result demonstrated that diamond-like carbon film exhibited the best adhesion to the mechanical heart valve. Secondly the structure and composition and topography of diamond-like carbon film on the mechanical heart valve were measured by the visible Raman spectroscopy and the X-ray photoelectron spectroscopy (XPS) and the atomic force microscopy (AFM). Finally human umbilical cord vein endothelial cells (HUVEC) were seeded on diamond-like carbon film on the mechanical heart valve and cultured in vitro. Raman spectroscopy revealed that disorder peak was at 1341.66 cm-1and graphite peak was at 1528.17 cm-1. XPS spectroscopy revealed that there was 21.45% of sp3 in the film. AFM revealed that the roughness average of the film was 26.4 nm. Human umbilical cord vein endothelial cells could proliferate on diamond-like carbon film very well. The result demonstrated that diamond-like carbon film on the mechanical heart valve deposited by pulsed laser deposition had good cell compatibility and could be covered with human umbilical cord vein endothelial cells fully so that it is possible to avoid cruor on the surface of the mechanical heart valve.
罗乐, 夏天荣, 方晓东, 严中亚, 程光存. 脉冲激光沉积类金刚石膜和细胞相容性研究[J]. 中国激光, 2009, 36(10): 2625. Luo Le, Xia Tianrong, Fang Xiaodong, Yan Zhongya, Cheng Guangcun. Experiment of Diamond-Like Carbon Film Deposited by Pulsed Laser and Cell Compatibility of Diamond-Like Carbon Film[J]. Chinese Journal of Lasers, 2009, 36(10): 2625.