采用405 nm发光二极管(LED)作为激发光源,选用孟加拉红(RB)作为光敏剂,可对早期口腔肿瘤进行诊断。正常的口腔黏膜不会被孟加拉红染色,而当口腔产生癌前病变或者生成肿瘤时,孟加拉红就会将病变部位染色。通过光谱测量可知,被染色病变部位的特征荧光光谱波长范围为570-600 nm,而正常口腔组织的特征荧光光谱中心波长约在480 nm。当癌前病变发展成恶性肿瘤之后,其特征荧光光谱发生改变,在630 nm和690 nm处各有一个典型的卟啉峰。以此为依据并结合光致荧光技术,采用荧光比例法,可以对口腔癌前病变进行诊断,灵敏度和特异性分别可达到95%和92.5%。这种无创、快速、早期的诊断方法,可以显著提高口腔癌患者的存活率。

405 nm LED was chosen as excitation source,Rose Bengal (RB) was used as photosensitizer,early oral tumor can be detected. Normal oral mucosa can not be dryed by Rose Bengal,oral precancerous lesions or tumors can be dryed by RB. It is known that characteristic fluorescence spectroscopy of lesions which were dryed ranged from 560 nm to 600 nm through spectral measurement,however normal oral cavity has special fluorescence peak around 480 nm. When precancerous lesions become malignant tumor,the special fluorescence spectroscopy was changed. Typical fluorescence peaks of porphyrin appeared at 630 nm and 690 nm. Combined with the technology of light induced fluorescence and the method of fluorescence ratio,oral precancerous lesions can be diagnosed,the sensitivity and specificity can arrive 95% and 92.5%. This is a non-invasive,quick and early diagnosis method. It increases survival rate of oral cancer patients remarkably.