近红外飞秒激光脉冲持续时间短、瞬时功率大、聚焦尺寸小和低吸收的特点,在细胞生物学领域有着广阔的应用前景。简述了3种不同类型的飞秒激光在细胞手术过程中的作用机理,介绍了飞秒激光手术在细胞生物学领域中的一些研究成果,如细胞骨架动力学、活细胞内细胞器手术、细胞膜工程、光动力学、生物活体实验及相应的应用。

Near-infrared femtosecond laser has broad application in the cell biology because of short pulse duration,high instantaneous power,small focus size and low absorption. The paper described three different types mechanism of femtosecond laser surgery briefly,and then introduced achievements of the femtosecond laser surgery in cell biology,such as cytoskeletal dynamics,ablation of organelles in living cells,engineering of the cell membrane,photodynamics,biological experiments in vivo and the corresponding applications.