采用傅里叶变换红外光谱法(FTIR),分析研究了肉苁蓉种子不同部位及其在不同处理后的光谱特征和成分差异。结果显示,肉苁蓉种子成分组成丰富,同时存在蛋白质、脂类和碳水化合物的特征吸收峰,并且推测含有果胶质和芳香类化合物。肉苁蓉种皮和种仁的成分差异很大,2 926,1 746,1 161及721 cm^-1 处一系列强峰说明种仁中的油脂类成分含量非常高,而种皮中1 054 cm^-1 处碳水化合物和果胶质类的特征吸收峰最强。霉变或高温处理后肉苁蓉种子中与活力相关的蛋白质及糖类含量明显减少,而有活力的种子中蛋白质与脂类所在特征区域的峰高比(I 1 630 /I 1 745 )(cm^-1 )均大于1.05。而肉苁蓉种仁失活后酰胺Ⅰ带与脂类特征峰的强度比值(I 1 653 /I 1 745 )由0.31降为0.23,降幅达25.8%。研究表明,红外光谱法不仅可应用于肉苁蓉种子成分的全面快速分析,而且还可更深入应用于种子生活力、种子寿命乃至种子质量的快速分析与测定。

Comparative study of the different parts of cistanche deserticola seeds and their changes after different processing were examined by Fourier transform infrared spectroscopy spectra (FTIR).The results of the analysis showed that components in the cistanche deserticola seeds were abundant,which contained characteristic absorption peaks of protein,fat and carbohydrate.As well,pectin and aromatic compound can be also found in the seeds.However,the components were different in different parts of cistanche deserticola seeds.The characteristic absorption peak intensities of fat at 2 926, 1 746,1 161 and 721 cm^-1 were the strongest in the seed kernels.However,the seed coats mainly consisted of carbohydrate and pectin,which were showed at 1 054 cm^-1 .The contents of protein and carbohydrate were decreased distinctly in the moldy and dead seeds after processing.The characteristic absorption peak intensity ratio of protein to fat (I 1 630 /I 1 745 ) was all higher than 1.05 in the live seeds.The characteristic absorption peak intensity ratio of amido link I of protein to fat (I 1 653 /I 1 745 ) in the dead seed kernels of the cistanche deserticola was decreased from 0.31 to 0.23,which was 25.8% less than that in vital seed kernels.The results suggest that FTIR not only can be used in fast comprehensive analysis of seed components,but also can be used in the seed vigor analysis,seed longevity determination and seed quality evaluation.