采用光谱分析技术对益生菌中植物乳杆菌的吸收和荧光光谱特性进行分析,并应用于益生菌的研究以及菌种鉴别。研究发现,植物乳杆菌对紫外波段的光有明显的吸收,峰值位于215 nm,在280 nm附近有一个对吸收光谱产生扰动的肩峰。对植物乳杆菌的荧光光谱检测发现,该菌种吸收紫外光后发射荧光。对荧光强度和菌液浓度关系的进一步研究,揭示了峰值强度随浓度变化的规律,并根据其变化规律,进行数据拟合,给出了相应的函数表达式。

The absorption spectra and fluorescence spectra of lactobacillus plantarum are measured and studied by spectral analysis technique,and the results will be applied to the study and classification of probiotic bacteria. The results show that lactobacillus plantarum obvioushy absorbs ultraviolet rays and emits obvious fluorescence. The peak of aborbtion spectra is at 215 nm,and there is a shoulder peak at 280 nm which disturb the absorbtion spectra. The studies of the spectra show relevant information in cells about lactobacillus plantarum. The relations between fluorescence intensities and the concentrations are also studied and the change law of intensity with increasing concentration is found. The functions of data fitting based on the law are shown.