Chinese Optics Letters, 2010, 8 (3): 309, Published Online: Mar. 11, 2010
Tagged molecule induced nanoparticle aggregation:Raman reporter-labeled immuno-Au aggregate as immuno-sensor Download: 734次
表面增强拉曼散射 免疫传感器 聚集体 拉曼探针 160.4236 Nanomaterials 290.5860 Scattering, Raman 300.6450 Spectroscopy, Raman
Abstract
A novel structure with high surface enhanced Raman scattering (SERS) activity and bio-specificity as a SERS-based immuno-sensor (named as Raman reporter-labeled immuno-Au aggregate) is demonstrated and employed for protein detection. In each fabrication process, the features of those aggregates are obtained and characterized by ultraviolet-visible (UV-Vis) extinction spectra, transmission electron microscopy (TEM) images, scanning electron microscopy (SEM) pictures, and SERS spectra. Experimental results indicate that proper amounts of the reporter molecules can result in the moderate aggregation morphologies of gold nanoparticles. Compared with the previously reported method using Raman reporterlabeled immuno-Au nanoparticles, more sensitive SERS-based protein detection is realized with this novel immuno-sensor.
Chunyuan Song, Zhuyuan Wang, Ruohu Zhang, Jing Yang, Xuebin Tan, Yiping Cui. Tagged molecule induced nanoparticle aggregation:Raman reporter-labeled immuno-Au aggregate as immuno-sensor[J]. Chinese Optics Letters, 2010, 8(3): 309.