激光技术, 2009, 33 (6): 561, 网络出版: 2010-03-11
石英微通道内测量细胞表面电荷的研究
Study on measuring surface-charges of cells in a quartz microchannel
医用光学与生物技术 细胞表面电荷 光镊 石英微通道 电泳 medical optics and biotechnology surface-charges of cells optical tweezers quartz microchannels electrophoresis
摘要
为了具体测量细胞表面电荷量,设计了一种利用光镊、微通道和电泳测量细胞表面电荷的方法,并利用该方法测量得到酵母菌细胞表面电荷大约为10-10C。结果表明,可以利用该方法测量细胞表面电荷变化。对测量系统进一步开发,可做成测量细胞表面电荷的生物分析芯片。
Abstract
The surface-charges of cells are very important to study the vital characteristic of cells.Common methods only measure the changes of cell electrophoresis mobility (EPM) instead of the quantity of cell surface-charges.In order to measure the surface-charges of cells,a novel method to measure the surface-charges of cells by means of optical tweezers and electrophoresis in a microchannel was designed.With this method,the quantity of the electric charge of yeast cells was measured about 10-10C.Experimental results prove that this method can be used to measure the changes of cell surface-charges.A lab-on-chip (LOC) for measuring the surface-charge of cells might be developed after improving this measuring system.
陈德良, 冯彩玲, 秦水介. 石英微通道内测量细胞表面电荷的研究[J]. 激光技术, 2009, 33(6): 561. CHEN De-liang, FENG Cai-ling, QIN Shui-jie. Study on measuring surface-charges of cells in a quartz microchannel[J]. Laser Technology, 2009, 33(6): 561.
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