采用一种抛物线形光纤针作为基本器件，设计制作了单光纤光镊系统.用时域有限差分（Finite Difference Time Domain，FDTD）的方法仿真了抛物线型光纤探针的出射场，并在稳态场下通过对麦克斯韦应力张量积分求出不同介质球半径和折射率情况下，介质球受到的横向和纵向光作用力.实验中此单光纤光镊系统实现了对水中酵母细胞的空间捕获，并且结合两个抛物线形光纤针实现了酵母细胞在两个光势阱中的转移交接.这种单光纤光镊系统结构简单、紧凑，操纵灵活，便于调整.可以适应更多的生物细胞和生物分子的光微操作需求.

A novel tapered fiber probe was proposed and fabricated using heating and drawing method.With this probe,a single fiber optic tweezers was constructed.The Finite Difference Time Domain (FDTD) method was used to calculate the output optical field of the tapered single fiber optical tweezers.The transverse and axial trapping forces were then obtained by integrating the Maxwell stress tensor in stable state.And the influences of different sizes and diffractive indexes microscopic particles on the optical force were considered.In the experiment,this single fiber optic tweezers successfully trapped a yeast cell in 3D.The trapped yeast cell can be transferred in two optical potential well of two tapered fiber optic tweezers.This fiber optic tweezers system is simple,compact,flexible and easily to be adjusted.