利用傅里叶变换红外光谱仪(FTIR)测定一定重金属浓度梯度处理下水芹和黄菖蒲根、 茎、 叶的红外光谱, 并对这些红外光谱进行了比较分析。 结果表明： 植物各组分吸附重金属前后的峰形基本不变, 只有某些参与重金属吸附的官能团如羟基(3 328～3 361 cm-1)、 羧基(1 402～1 440 cm-1)、 酰胺基(1 620～1 645 cm-1)等的吸收峰发生了不同程度的位移。 两种水生植物根中与植物生理有关的化学成分的特征峰大多数随重金属浓度增加而降低。 水芹茎、 叶在各浓度重金属处理下与植物生理有关的化学成分的特征峰大多数都比对照低, 黄菖蒲则相反。 综合分析, 黄菖蒲对重金属废水的修复效果和耐受能力均强于水芹。 采用FTIR法鉴别水生植物对重金属的处理效果比常规方法更具代表性, 为植物修复的开展提供了有力的证据支持。

The spectra of root, stem, leaf of two hydrophytes (Oenanthe javanica and Iris pseudacorus) with CK, low, medium and high metal treatment (Cr, Fe, Mn and Zn) were determined directly, fast and accurately by Fourier transform infrared (FTIR) spectrometry with OMNI-sampler. The results showed that the peak shape of plant component remained invariable basically when absorbing heavy metals. Significant absorption peak shifts of some functional groups such as hydroxy groups (3 328-3 361 cm-1), carboxyl groups (1 402-1 440 cm-1), and acidamide groups (1 620-1 645 cm-1)can be seen when they participated in the process of absorbing heavy metals. The varieties of organic characteristic peaks were also capable of reflecting physiology and metabolism changes. With increasing heavy metal concentration, the absorption intensity of most root characteristic peaks correlated with physiology decreased significantly for the two hydrophytes, but it was decreased for stem, leaf characteristic peaks of Oenanthe javanica. The absorbance of stem, leaf was bigger than the control for different heavy metals for Iris pseudacorus. To sum up, although two hydrophytes could endure certain concentration of heavy metals, the tolerance of Iris pseudacorus was greater than that of Oenanthe javanica. With strong identifying ability, exact determination results and fast response function, the application of FTIR method has been popularized gradually in the field of environmental science. Recently, greater progress has been made in the study and use of FTIR in the fields of environmental accident emergent monitoring, environmental monitoring and environmental chemistry research. The infrared spectra could also be widely used to monitor changes in chemical composition of plant parts under stresses and environmental restoration and supply the best evidence for phytoremediation. This method was an important tool for detecting unknown pollutants and environmental monitoring.