光谱学与光谱分析, 2009, 29 (6): 1558, 网络出版: 2010-05-26
红外光谱法对双黄连粉针剂的定性定量分析
Qualitative and Quantitative Analysis of Shuanghuanglian Fenzhenji by FTIR
摘要
通过分析双黄连粉针剂的红外光谱并与对照品进行比较, 初步指认了粉针剂中各类成分的吸收峰, 为定性质量分析提供了依据。 建立了基于中红外光谱的黄芩苷与绿原酸含量校正模型。 模型的R2值均在0.99以上, 平均预测相对误差也低于4%, 具有良好的拟合及预测能力。 本研究结果说明, 使用红外光谱法对双黄连粉针剂进行在线定性定量分析, 方便快速, 切实可行。
Abstract
Qualitative and quantitative analysis of Shuanghuanglian fenzhenji was studied in the present paper by FTIR. The fingerprint characteristics of main components in the injections were assigned and can be used as the criterion in the qualitative quality control. Calibration models by PLS algorithm were built to get the quantitative information of baicalin and chlorogenic acid in the injection samples. The R2 of calibration models are both above 0.99 and the average relative deviations between predicted and actual value measured by HPLC are less than 4% for the both properties. The qualitative and quantitative analysis by FTIR is simple and rapid, so it may provide a new on-line approach to the quality control of main constituent in Shuanghuanglian fenzhenji and some other traditional Chinese medicines.
阎姝, 徐茂玲, 图雅, 李惠芬, 孙素琴. 红外光谱法对双黄连粉针剂的定性定量分析[J]. 光谱学与光谱分析, 2009, 29(6): 1558. YAN Shu, XU Mao-ling, TU Ya, LI Hui-fen, SUN Su-qin. Qualitative and Quantitative Analysis of Shuanghuanglian Fenzhenji by FTIR[J]. Spectroscopy and Spectral Analysis, 2009, 29(6): 1558.