利用溶胶凝胶法制备了用有机荧光染料2,5-双(5-叔丁基-1,3-苯并噁唑-2-基)噻酚(2,5-bis(5-tert-butyl-2-benzoxazolyl)thiophene, BBOT)的高浓度掺杂的纳米多孔二氧化硅固态薄膜， 该薄膜呈现明亮的蓝色发光。 通过紫外-可见光谱、 瞬态-稳态荧光光谱、 光学显微镜技术的研究表明： (1)在掺杂浓度低于6×10-3 mol·L-1时， 薄膜中荧光染料BBOT的光强比值随浓度呈线性增加； (2)在掺杂浓度低于6×10-3 mol·L-1时， 显微镜观察可见薄膜发光均匀、 没有相分离产生； (3)固体薄膜中分子的荧光寿命比在1,4二氧六环稀溶液中的1.957 ns延长。 在掺杂浓度为6×10－3　mol·L-1的样品中随着凝胶化程度逐渐升高荧光寿命从固化温度为50　℃样品的2.45 ns提高到固化温度为90　℃样品的3.04 ns。　在掺杂浓度高达6×10－3　mol·L-1时， 该体系并不产生分子荧光的浓度猝灭。 通过对比固态掺杂体系中和相同浓度下1,4二氧六环溶液中BBOT的荧光寿命随浓度和二氧化硅体系凝胶化程度变化的规律， 发现多纳米孔二氧化硅基质可以有效地抑制BBOT荧光的浓度猝灭， 获得了具有稳定荧光的有机无机杂化材料。

Thin nano-porous silica films doped with high concentrations of fluorescent material, 2,5-bis(5-tert-butyl-2-benzoxazolyl)-thiophene (BBOT) were prepared via a sol-gel process. Uniform and bright blue fluorescence was observed. Light emission properties of these organic molecule doped inorganic silica films, i.e., hybrid films, were measured using ultraviolet-visible (UV-Vis) absorption spectroscopy, steady and time-resolved fluorescence spectroscopy as well as optical microscopy. Features of these materials were revealed in this investigation: Firstly, photoluminescence intensity from BBOT doped silica films increased linearly as the concentrations of BBOT increased if the dopant concentration was relatively low and below 6×10-3 mol·L-1; Secondly, no molecular aggregation or phase separation was observed using optical microscopy when the BBOT concentration was below 6×10-3 mol·L-1 in BBOT doped silica films. Thirdly, the fluorescence lifetimes of BBOT in the doped silica films were longer than that in a dilute dioxane solution (1.957 ns), which was contradicted to our general understanding that the fluorescence lifetime may be reduced in a condensed matter due to molecular interactions or quenching. It was further found that the fluorescence lifetime also varied with the gelation conditions. Taking a BBOT concentration of 6×10-3 mol·L-1 for an example, the lifetime of BBOT in doped silica films was about 2.45 ns for a specimen polymerized at 50 ℃; while the lifetime was increased to 3.04 ns for a specimen polymerized at 90 ℃. This work demonstrates no concentration quenching when the BBOT dopant concentrations increased to as high as 6×10-3 mol·L-1 in the silica matrix. In comparison with the changes in time-resolved photoluminescence of BBOT in dioxane solution and that of the BBOT doped nano-porous silica in relation to their concentration dependence and the gelation conditions, it was found that concentration quenching can be effectively suppressed by the nano-porous silica matrix. A stable fluorescent organic-inorganic hybrid material is thus obtained.