光谱学与光谱分析, 2009, 29 (7): 1844, 网络出版: 2010-05-26  

太空育种射干的FTIR分析

Analysis of Belamcanda Chinensis with Space Flight Mutagenesis by FTIR
作者单位
1 燕山大学理学院, 河北 秦皇岛066004
2 燕山大学电气工程学院, 河北 秦皇岛066004
摘要
对我国首创的第4代太空育种射干与地面组射干的非晶成分从整体上进行了测定和对比分析, 以期为更全面地了解太空射干品质的变化和深入研究积累资料, 探索建立太空射干质量标准。 结果表明两组样品的FTIR谱的主要吸收峰的峰位、 峰形大致相同, 但峰的强度却有明显差异。 与地面组相比, 太空射干在1 642 cm-1处酮类的CO伸缩振动峰增强, 表明太空射干中的主要活性成分异黄酮类化合物含量明显增加, 在1 318 cm-1处一水草酸钙吸收峰增加, 在1 541, 1 456 cm-1处分子中苯环的环伸缩振动峰、 1 417 cm-1处亚甲基的C—H键弯曲振动峰以及1 051 cm-1处伯醇的C—O的伸缩振动峰都明显减弱。 太空诱变育种有利于筛选出活性成分含量提高的射干新品种。 预期太空射干的药效比地面组的有所增强, 有待于深入研究来证实。
Abstract
The amorphous active components of the space mutagenesis Belamcanda chinensis and the ground group were measured, compared and analyzed. The purpose was to get a comprehensive understanding of the changes in quality of the 4th generation space mutagenesis Belamcanda chinensis, accumulate data for further studies, and try to establish the quality criterions of space mutagenesis Belamcanda chinensis. The result shows that the FTIR spectra of the space sample are almost the same as that of the ground one in terms of the main absorption peaks positions and shapes, but there are obvious differences in intensities. The intensity of the absorption peak at 1 642 and 1 318 cm-1 of the space group was remarkably enhanced than the ground group, but at 1 541, 1 456, 1 417 and 1 051 cm-1 it was decreased compared to the ground group. At the same time, the peak at 1 642 cm-1 of the stretching vibration of CO, the characteristic absorption of the keto, was remarkably enhanced. The peaks at 1 541 and 1 456 cm-1 were assigned to C—C groups, the peak at 1 417 cm-1 was due to the —CH2— groups, the peak at 1 318 cm-1 was the characteristic absorption of calcium oxalate monohydrate, and the peak at 1 051 cm-1 was assigned to C—O groups. It was shown that the relative content of flavone was increased distinctly. Space mutation breeding is conducive to breeding new varieties of highly active ingredients, it is also one of the ways to innovate germplasm resources of Chinese medicines efficiently. The effect of the space group is expected to be enhanced than the ground group, but it needs to be proved through further research .

张红梅, 关颖, 史锦山. 太空育种射干的FTIR分析[J]. 光谱学与光谱分析, 2009, 29(7): 1844. ZHANG Hong-mei, GUAN Ying, SHI Jin-shan. Analysis of Belamcanda Chinensis with Space Flight Mutagenesis by FTIR[J]. Spectroscopy and Spectral Analysis, 2009, 29(7): 1844.

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