激光技术, 2008, 32 (5): 0490, 网络出版: 2010-06-03
共振喇曼光谱法检测人体皮肤中类胡萝卜素
Study of carotenoid in human skin by means of resonance Raman detection
摘要
类胡萝卜素作为一种强抗氧化剂在人体的抗氧化防御系统中起着重要作用,它在人体中的含量可以用来表征人体的健康状况。为了研究人体中的类胡萝卜素含量,采用了共振喇曼光谱法检测人体皮肤中的类胡萝卜素。经过反复实验得到了实验参量的最佳选择。例如选择测量部位为拇指内侧,入射波长473nm、入射光强度20mW、曝光时间5s。同时通过甘油匹配的方法增加了探测到的喇曼信号的强度。利用拟合差分算法有效去除了荧光背景噪声。最终获得了明显的人体皮肤中类胡萝卜素的喇曼光谱。结果表明,用共振喇曼光谱法检测人体皮肤中类胡萝卜素是一种行之有效的方法。
Abstract
Carotenoid molecules are powerful antioxidants that play important role throughout the human body. The level of carotenoid in human body can be used to reflect the human's health condition. Experiments were carried to detect the carotenoid in human. After tests repeatedly,suitable experimental parameters were determined. That is to say the best location to perform the measurement is thrum,laser's wavelength is 473nm,intensity of laser is 20mW and exposure time is 5s. At last obvious resonance Raman spectra of carotenoid in human skin was obtained after the fluorescence background was fitted and subtracted from the original spectra by means of fitted differential algorithm. Experimental results prove that resonance Raman spectra is applicable for carotenoid detection in human.
南楠, 邵永红, 姜耀亮, 檀慧明. 共振喇曼光谱法检测人体皮肤中类胡萝卜素[J]. 激光技术, 2008, 32(5): 0490. NAN Nan, SHAO Yong-hong, JIANG Yao-liang, TAN Hui-ming. Study of carotenoid in human skin by means of resonance Raman detection[J]. Laser Technology, 2008, 32(5): 0490.