基于血红蛋白、肌红蛋白等色素在近红外波段的吸收谱,采用近红外光谱(NIRS)技术,通过检测出射光相对入射光的衰减,可解算生物组织中上述色素的浓度。 基于稳态空间分辨的NIRS研发了可检测组织中色素浓度的装置,该装置的传感器包括一个可发射3个不同波长的LED光源,以及与LED位于同一直线上且距离分别为30和40mm的２个检测器。 用该装置检测了2块猪肉组织(分别为新鲜的猪背长肌和股直肌)的色素浓度,结果表明,背长肌和股直肌中血红蛋白、肌红蛋白等色素的总浓度分别为(6.42±1.51)μmol·L-1和(15.48±4.54)μmol·L-1,接近于现有报道的经验值。 在无损、实时、直接、简便检测猪肉组织的色素浓度方面,上述NIRS方法及装置是可信的。

Based on the absorption spectra of hemoglobin and myoglobin in the near infrared band,the concentrations of these pigments in the biological tissues can be obtained using near infrared spectroscopy (NIRS) by detecting the intensity attenuation of the emitted light compared with the incident light. Based on the steady-state spatially resolved NIRS,the prototype for detecting the concentrations of tissue hemoglobin and myoglobin was independently developed by our group. The probe consisted of an LED light source which could emit three different wavelengths in the near infrared band,and two detectors which were placed on the same line with and at the distances of 30/40mm to the LED. The pigment concentrations of two pieces of pork,one from the erector spinae and the other from the rectus femoris,were detected using this prototype. The total concentrations of hemoglobin and myoglobin (ctotal) were (6.42±1.51)μmol·L-1 in the erector spinae,and (15.48±4.54)μmol·L-1 in the rectus femoris,respectively. The myoglobin was dominant in both of the results. These were consistent with the recent empirical reports. In summary,the NIRS method and prototype are authentic in detecting the pigment concentrations of pork tissue non-invasively,real-time,directly and conveniently.