采用傅里叶变换红外光谱(FTIR)结合二阶导数谱和热扰动下的二维相关红外光谱技术对6种不同花粉, 即杏花花粉、 油菜花粉、 茶花花粉、 西瓜花粉、 荷花花粉和虞美人花粉, 进行了快速无损的鉴别。 结果表明, 在一维红外光谱图上, 不同花粉的蛋白质、 脂肪和糖类物质的特征吸收峰在相对峰强和峰位上均存在一定的差异, 在二阶导数谱上差异很明显。 而在二维红外谱图上, 由于6种花粉的自动峰及相关峰峰簇的位置和数量不同, 其差别体现得更为明显和直观。 因此, 三级红外宏观指纹图谱法是鉴别不同蜂花粉种类的一种有效和快速检测方法。

Six kinds of bee pollens, including apricot pollen, lotus pollen, rape pollen, camellia pollen, watermelon pollen and corn poppy pollen, were identified non-destructively by Fourier transform infrared spectroscopy (FTIR) combined with derivative spectra and two-dimensional correlation spectroscopy (2D) in the present article. Compared with conventional IR spectra of samples, some certain differences were found in the characteristic peaks of proteins, lipids and carbohydrates. Obvious differences of the six kinds of bee pollens were found in the second derivative spectra. And in the 2D-IR correlation spectra, the samples presented the differences in the position and intensity of the autopeaks and correlation peak clusters. Therefore, the three-step IR macro-fingerprint provides a more rapid and effective method for the identification of different kinds of bee pollens.