采用光镊技术研究了离体血红细胞的动力学过程即细胞活性的变化规律。当光镊中的半导体激光器发出的高斯光束照射红细胞使其发生形变并反转,待细胞保持动态平衡后切断激光光路,得到形变血红细胞的自由回复时间,该时间长短与细胞活性相关联。通过对比被小功率He-Ne激光器持续照射和正常离体血液的活性衰变规律,结果表明,细胞活性均呈现出规律性的变化,波动之后逐渐减弱并最终趋向死亡。被激光持续照射的红细胞活性衰变较缓,即激光照射的作用延缓了红细胞的衰弱。研究结果能为细胞的活性和动力学分析提供参考,为血液疾病的临床检测和激光临床治疗提供帮助。

Optical tweezers were used to study the dynamic process and the changing rule of the activity of the red blood cells in vitro.Red blood cells irradiated by the Gaussian beam of LD laser were deformed and reversed in optical tweezers.When the LD laser was cut off,the free recovery time of blood cells was obtained,which was associated with the cells′ activity.By comparing the decay process of cells activity between irradiated the red blood cells by low-power He-Ne laser and static red blood cells,the results showed:the cells activity presented a changing rule that,after fluctuations,it gradually declined till cells died.Irradiated by low-power He-Ne laser,red blood cells activity decreased slowly,namely laser irradiation postponed some decay stages.The results can provide valuable reference for the study of cells viability and dynamic analysis.