Chinese Optics Letters, 2010, 8 (10): 937, Published Online: Oct. 19, 2010
Spectral imaging of time-resolved anisotropy: theory and experiment Download: 606次
荧光显微镜 荧光时间传感 光谱、荧光和发光 170.2520 Fluorescence microscopy 170.3650 Lifetime-based sensing 170.6280 Spectroscopy, fluorescence and luminescence
Abstract
Time-resolved fluorescence anisotropy on the nanosecond time scale is useful for the study of the rapid rotation of macromolecules. A system combining the capabilities of fluorescence spectral imaging with time-resolved fluorescence anisotropy and enabling the wide-field measurement of the spectroscopic parameters of fluorophores is discussed. The phasor approach is used to quantitatively analyze the time-resolved fluorescence anisotropy by transforming the polarized parallel and perpendicular components to the phasor space in the frequency domain, respectively, and a unique way to calculate the fluorescence rotational correlation time is put forward. Experimental results prove that the phasor approach is a proper model for the time-resolved fluorescence anisotropy.
Yanzhou Zhou, Qingruo Wang, Jingsong He, Lerong Lin. Spectral imaging of time-resolved anisotropy: theory and experiment[J]. Chinese Optics Letters, 2010, 8(10): 937.