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Circulation times of hepatocellular carcinoma cells by in vivo flow cytometry

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Abstract

Hepatocellular carcinoma (HCC) may metastasize to many organs. The survival rate is almost zero for metastatic HCC patients. Molecular mechanisms of HCC metastasis need to be understood better and new therapies must be developed. We have developed the "in vivo microscopy" to study the mechanisms that govern liver tumor cells spreading through the microenvironment in vivo. A recently developed "in vivo flow cytometer" combined with real-time confocal fluorescence imaging is used to assess spreading and the circulation kinetics of liver tumor cells. We measure the depletion kinetics of two related human HCC cell lines, high-metastatic HCCLM3 cells and low-metastatic HepG2 cells, which are from the same origin and obtained by repetitive screenings in mice. More than 60% of the HCCLM3 cells are depleted within the first hour. Interestingly, the low-metastatic HepG2 cells possess noticeably slower depletion kinetics. In comparison, less than 40% of the HepG2 cells are depleted within the first hour. The differences in depletion kinetics might provide insights into early metastasis processes.

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收稿日期:2010-07-07

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网络出版日期:0001-01-01

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李延:Department of Chemistry, Fudan University, Shanghai 200433, ChinaInstitutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
樊志超:Department of Chemistry, Fudan University, Shanghai 200433, ChinaInstitutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
郭进:Department of Chemistry, Fudan University, Shanghai 200433, ChinaInstitutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
刘光大:Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, ChinaShanghai Medical College, Fudan University, Shanghai 200032, China
谭晓英:Department of Chemistry, Fudan University, Shanghai 200433, ChinaInstitutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
王成:School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
顾正勤:Department of Urology, Xinhua Hospital, Shanghai Jiaotong University, Shanghai 200092, China
魏勋斌:Department of Chemistry, Fudan University, Shanghai 200433, China

联系人作者:联系作者(xwei@fudan.edu.cn)

备注:This work was partially supported by the National "973" Program of China (No. 2011CB910400), the China National Key Projects for Infectious Disease (No. 2008ZX10002-021), the National Science Foundation of China (Nos. 30770524 and 20975027), the Ministry of Education of China (No.109056), "Shuguang Scholarship" from the Education Commission of Shanghai Municipality, and the Program for New Century Excellent Talents in University Award (NCET-08-0131).

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引用该论文

Yan Li, Zhichao Fan, Jin Guo, Guangda Liu, Xiaoying Tan, Cheng Wang, Zhengqin Gu, Xunbin Wei, "Circulation times of hepatocellular carcinoma cells by in vivo flow cytometry," Chinese Optics Letters 8(10), 953-956 (2010)

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