中国激光, 2010, 37 (11): 2730, 网络出版: 2010-11-16
CdSe/CdS/ZnS量子点对体外培养成熟卵母细胞的侵入性研究
Invasion of CdSe/CdS/ZnS Quantum Dots for Oocytes in Vitro Maturation
生物光学量子点 卵母细胞 体外培养成熟 侵入性 生殖毒性 biotechnology quantum dot oocytes in vitro maturation invasion reproductive toxicity
摘要
为了研究CdSe/CdS/ZnS荧光量子点的生殖毒性, 通过建立雌性昆明小鼠卵母细胞体外培养成熟体系, 将量子点加入到卵母细胞培养液, 使其浓度为28.90 nmol/L, 于37 ℃,体积分数为5%的CO2和饱和湿度下分别培养4, 8和20 h, 观察卵母细胞形态并在相差荧光显微镜下拍照统计。实验结果表明, 在该剂量量子点作用下, 随培养时间延长, 进入颗粒细胞的量子点增加, 并累积于细胞膜附近, 但未发现量子点进入卵母细胞内部, 激光共聚焦荧光显微镜的高分辨率层析图证明了该结论。在该剂量量子点作用20 h后, 量子点虽未进入卵母细胞, 但卵母细胞的成熟率显著下降。
Abstract
The Kunming mice immatured oocytes in vitro maturation culture system are established to investigate the reproductive toxicity of CdSe/CdS/ZnS quantum dots (QDs). QDs stock solution is added into oocyte culture medium at a final concentration of 28.90 nmol/L. Then, QDs and oocytes are co-cultured at 37 ℃, 5% CO2 for 4, 8 and 20 h, respectively. The morphological information of oocytes are observed and analyzed under phase-contrast fluorescence microscope. The results demonstrate that QDs enter cumulus cells and accumulate with co-culture time. QDs can not penetrate oocytes zona pellucida, which is confirmed by laser scanning confocal microscope with high spatial resolution. After being treated for 20 h and being rejected by oocytes, QDs decrease the ratio of oocyte in vitro maturation dramatically.
王晓梅, 杨坚泰, 许改霞, 林晓潭, 周消清, 屈军乐, 陈思平, 牛憨笨. CdSe/CdS/ZnS量子点对体外培养成熟卵母细胞的侵入性研究[J]. 中国激光, 2010, 37(11): 2730. Wang Xiaomei, Yong Ken-Tye, Xu Gaixia, Lin Xiaotan, Zhou Xiaoqing, Qu Junle, Chen Siping, Niu Hanben. Invasion of CdSe/CdS/ZnS Quantum Dots for Oocytes in Vitro Maturation[J]. Chinese Journal of Lasers, 2010, 37(11): 2730.