光学学报, 2010, 30 (12): 3563, 网络出版: 2010-12-07
显微干涉术在血红细胞光相位场定量测量中的应用
Application of Microscopic Interferometry for Quantitative Phase Measurement of Red Blood Cells
医用光学 相位分布 显微干涉 血红细胞 定量测量 medical optics phase distribution microscopic interferometry red blood cell quantitative measurement
摘要
为提高生物细胞形态检测速度和精度,以典型相位物体血红细胞为研究对象,结合离轴干涉、共焦显微和高速图像采集技术,提出了细胞光相位场定量测量方法,既有高测量精度又可以对流动的细胞进行实时放大观测。实验中采集单幅载频干涉图,运用快速傅里叶变换相位提取法和路径无关相位解包算法,最终获得血红细胞光相位场的定量分布,和已有的光学相位模型吻合得较好。分析表明,该系统分辨率达到亚微米量级。这种由细胞光相位场分布解构细胞形态的检测手段希望成为一种新的生物活体细胞实时在体检测和定量分析的方法。
Abstract
In order to improve detection speed and accuracy of biological cells, microscopic interferometry which is a noncontact technique for imaging of phase objects is proposed, i.e., red blood cells. The technique combines the principles of off-axis interferometry, confocal microscopy and high-speed image-capture technology and is characterized by optimized spatial resolution and real-time acquisition capabilities. Single carrier-frequency interferogram is captured from experimental setup. According to fast Fourier transform phase retrieval method and path-unfollowing unwrapping algorithm, the quantitative phase distributions of red blood cell are gained and agree well with the previous optical phase models. Analysis shows that the resolution of introduced system reaches sub-micron dimension. It provides a breakthrough method for real-time observing and quantitative analyzing of cells in vivo.
薛亮, 来建成, 王绶玙, 李振华. 显微干涉术在血红细胞光相位场定量测量中的应用[J]. 光学学报, 2010, 30(12): 3563. Xue Liang, Lai Jiancheng, Wang Shouyu, Li Zhenhua. Application of Microscopic Interferometry for Quantitative Phase Measurement of Red Blood Cells[J]. Acta Optica Sinica, 2010, 30(12): 3563.