以3种不同耐热型玉米植株叶片为样品，分析了在10~50 ℃温度胁迫下，样品离体叶片延迟荧光光谱的变化。实验发现：温度对延迟荧光光谱主峰685 nm的最高峰值强度(F685)和次峰730 nm的最高峰值强度(F730)的比值F685/F730产生了显著的影响。此外，不同耐热型样品在10 ℃胁迫下的F685/F730值(V10)与50 ℃胁迫下的F685/F730值(V50)之间存在显著差异：不耐热样品叶片的V10/V50高于耐热的，越耐热的样品V10/V50值越小，且随着胁迫时间的延长，V10/V50也逐渐减小。因此，通过对实验结果的分析认为，延迟荧光光谱F685/F730比值是随着温度胁迫规律变化的，F685/F730比值的温度响应曲线有望成为一种鉴定和评价植物耐热性的新方法。

In order to obtain the relationship between temperature-stress and delayed fluorescence (DF) emission spectra, in this paper, three different heat-resistant maizes were used as samples to experimentally study the plant DF emission DF spectra at different elevated temperatures (10～50 ℃). The experimental spectra were obtained by 660 nm excitation. Results showed that there are two distinct emission peaks in DF spectra, that is, the main peak at around 685 nm and the second peak at around 730 nm. The maximal values of DF emission spectra at 685 nm and 730 nm were marked as F685 and F730, respectively. The experimental results illustrated that the F685/F730 ratio varied with the increase of temperature. It was also found that the F685/F730 ratio at 10 ℃and 50 ℃, which was marked as V10 and V50, respectively, are obviously different. The V10/V50 ratio depended on the heat-resisting property of the samples. These results indicated that the higher the heat-resisting property of the sample is, the lower the V10/V50 ratio is. Additionally, the longer temperature stress time is, the lower V10/V50 ratio is. Hence, we concluded that DF emission spectra vary with the temperature-stress, and the response curve of F685/F730 ratios of different samples display a similar law. Moreover, the responses of F685/F730 ratio of DF emission spectra to the temperature for the different heat-resistant Maize samples could be a new and useful judgment to identify and evaluate the heat-resisting property of maize.