电感耦合高频等离子体发射光谱法测定木耳膳食纤维多种微量元素的方法。 采用湿法酸消解制备样品, 硝酸∶高氯酸(10∶1), 120 ℃恒温消化溶解, 酸度控制<10%, 满足ICP-AES测定的要求。 经检测国家标准物质GBW07602验证, 方法的准确度在1.12%～6.15%之间, 精密度在0.41%～5.69%之间。 实验数据表明, 该方法是木耳中微量元素检测简便有效的方法。 同时应用扫描电子显微镜进行木耳膳食纤维表面结构测定, 并采用化学分析方法测定木耳膳食纤维中蛋白质、 粗脂肪、 粗纤维及水分的含量, 其相关性研究为木耳膳食纤维开发利用提供了新途径。

The simultaneous determination of trace elements in ultrafine agaric was carried out by inductively coupled plasma atomic emission spectrometry (ICP-AES). The experiments were done using wet acid digestion sample preparation. The samples were dissolved at 120 ℃ constant temperature. The requirements of determination by ICP-AES can be satisfied at an acidity controlled less than 10%. The method is simple, rapid and economic. National standard material GBW07602 was detected by authentication. Its accuracy is between 1.12% and 6.15. The precision is between 0.41% and 5.69%. At thesame time, the surface structure of ultrafine agaric powder was examined by scanning electron microscope. And the dietary fiber was determined for fungus protein, crude fat, crude fiber, moisture and ash content by chemical analysis. The study on external and internal relevance of agaric could provide a new approach to scientific research on the development and application of fungus dietary fiber.